《植物生理学报》 2012, 48(11): 1037-1042

环境条件对芽孢杆菌B-9987菌株防御酶诱导效应的影响

高伟^1,2, 田黎^1,2,*, 张久明², 郑立¹, 崔志松¹, 史振平²

1国家海洋局第一海洋研究所, 山东青岛 266061; 2青岛科技大学生物系, 山东青岛 266042

通信作者：田黎；E-mail: wshw8@yahoo.com.cn；Tel: 0532-88967423

摘　要：

研究室内外条件下, 海洋芽孢杆菌不同处理物对番茄防御酶系的诱导作用。分别使用不同浓度芽孢杆菌B-9987发酵 液和无细胞滤液喷施番茄植株, 于室内外两种环境条件下养殖, 测定了处理11 d内番茄5种防御酶的变化趋势。两种处理诱 导抗性结果显示, 在两种环境条件下处理植株苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)、过氧化物酶(POD)、过氧化氢酶 (CAT)较空白对照植株酶活性均有不同程度增加。除超氧化物歧化酶(SOD)外, 室内环境较室外更利于B-9987菌株处理液 对番茄防御酶系的诱导作用发挥; 相同环境条件下, 发酵液的处理诱导活性较强。B-9987菌株对番茄防御酶系有较好的诱 导作用, 但诱导作用的发挥受环境条件影响明显。

关键词：防御酶; 海洋芽孢杆菌; 诱导抗性

收稿：2012-07-23   修定：2012-09-28

资助：“十二五”国家高技术研究发展计划“863”重大项目(2011AA10A202-2和2011AA09070402)、“十二五”国家科 技支撑计划(2011BAE06B04)、青岛科技计划基础研究项 目(10-3-4-4-7-jch)和海洋公益项目(201005032-2)。

Inducing Effect of Defense Enzyme Activities by Bacillus marinus B-9987 under Different Environmental Conditions

GAO Wei^1,2, TIAN Li^1,2,*, ZHANG Jiu-Ming², ZHENG Li¹, CUI Zhi-Song¹, SHI Zhen-Ping²

¹First Institute of Oceanography, Qingdao, Shandong 266061, China; ²Biology Department, Qingdao University of Science & Technology, Qingdao, Shandong 266042, China

Corresponding author: TIAN Li; E-mail: wshw8@yahoo.com.cn; Tel: 0532-88967423

Abstract:

The comparative study on the activities of defense enzymes was obtained in tomato leaves under different environmental conditions. The tomato plants were sprayed with B-9987 fermentation broth and cell free filtrate. The activities of the 5 defense enzymes in tomato leaves were assayed. The trend of the enzymes activities in 11 days was analysed in the experiment. According to the result, the B-9987 extracts had an inducing effect on phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO), peroxidase (POD), catalase (CAT) in tomato leaves. The indoor treatment of the enzymes [except superoxide dismutase (SOD)] showed higher effect comparing outdoor treatment. The inducement of fermentation broth was much better than the cell filtrate. The result showed that the B-9987 extracts had an inducing effect on the defense enzymes in tomato. The environmental conditions greatly affect the inducement of B-9987 extracts.

Key words: defense enzymes; Bacillus marinus; induced resistance